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Cells and Tissues in Culture Methods, Biology and Physiology


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Oktober 2013

Beschreibung

Beschreibung

Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine.
The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research.
The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. The text also reviews invertebrate tissue and organ culture in cell research; introduction and methods employed in plant tissue culture; and growth, differentiation and organogenesis in plant tissue and organ cultures.
The selection is a vital source of data for readers interested in the culture of cells and tissues.

Inhaltsverzeichnis

1;Front Cover;1 2;Cells and Tissues in Culture: Methods, Biology and Physiology;4 3;Copyright Page;5 4;Table of Contents;12 5;Contributors;6 6;Foreword to Volume 3;8 7;Foreword to Volume 1;9 8;Foreword to Volume 2;10 9;Contents of Volume 1;16 10;Contents of Volume 2;17 11;CHAPTER 1. The Molecular Organization of Cells and Tissues in Culture;18 11.1;I. INTRODUCTION;18 11.2;II. METHODS;19 11.3;III. CELLULAR ORGANIZATION AT THE MOLECULAR LEVEL;21 11.4;IV. FlBROGENESIS IN TlSSUE CULTURE;48 11.5;REFERENCES;73 12;CHAPTER 2. Tissue Culture in Radiobiology;80 12.1;I. HISTORICAL INTRODUCTION;81 12.2;II. THE EFFECTS OF RADIATION ON CELL PROLIFERATION;83 12.3;III. EFFECTS OF RADIATION ON THE GROWTH OF TISSUE AND CELL CULTURES;85 12.4;IV. EFFECT OF RADIATION ON THE GROWTH OF ISOLATED CELLS;109 12.5;V. IRRADIATION OF SELECTED PARTS OF THE CELL;117 12.6;VI. SOME FALLACIOUS METHODS OF GROWTH MEASUREMENT;120 12.7;VII. NON-MITOTIG CELL DEATH;123 12.8;VIII. DEGENERATIVE CHANGES IN THE CYTOPLASM;128 12.9;IX. RADIATION AND CELL DIFFERENTIATION;130 12.10;X. THE RADIOSENSITIVITY OF MALIGNANT CELLS;132 12.11;XI. CHEMICAL FACTORS INFLUENCING RADIOSENSITIVITY;133 12.12;XII. EFFECTS OF RADIATION ON THE CULTURE MEDIUM;141 12.13;XIII. COMPARISON OF IN VITRO WITH IN VIVO SENSITIVITY;143 12.14;XIV. COMPARISON OF DIFFERENT SORTS OF IONIZING RADIATION;147 12.15;XV. EFFECTS OF ULTRAVIOLET RADIATION;151 12.16;XVI. CONCLUSION;152 12.17;REFERENCES;153 13;CHAPTER 3. Effects of Invading Organisms on Cells and Tissues in Culture;168 13.1;I. INTRODUCTION;169 13.2;II. PROTOZOA;172 13.3;III. BACTERIA;173 13.4;IV. MYGOPLASMA (PPLO);179 13.5;V. MlYAGAWANELLA;180 13.6;VI. POXVIRUSES;183 13.7;VII. HERPES VIRUSES;190 13.8;VIII. ADENOVIRUSES;198 13.9;IX. PAPOVA VIRUSES;203 13.10;X. MYXOVIRUSES;208 13.11;XI. AVIAN SARCOMAS AND LYMPHOMAS;221 13.12;XII. PIGORNA VIRUSES;230 13.13;XIII. ARBOVIRUSES;240 13.14;XIV. MISCELLANEOUS VIRUSES;245 13.15;XV. APOLOGIA AND EPILOGUE;250 13.16;REFERENCES;252 14;CHAPTER 4. Cell, Tis
sue and Organ Cultures in Virus Research;280 14.1;I. INTRODUCTION;280 14.2;II. ISOLATION OF VIRUSES IN TISSUE CULTURE;282 14.3;III. IDENTIFICATION OF VIRUSES;283 14.4;IV. TITRATION OF VIRUSES;301 14.5;V. REPLICATION OF VIRUSES;304 14.6;VI. VIRAL GENETICS;315 14.7;VII. PRODUCTION OF VIRUS VACCINES;318 14.8;VIII. TRANSFORMATION OF CELLS BY VIRUSES;320 14.9;IX. VIRUS STUDIES IN ORGAN CULTURES;321 14.10;REFERENCES;323 15;CHAPTER 5. Antibody Production in Tissue Culture;334 15.1;I. INTRODUCTION;334 15.2;II. EARLY ATTEMPTS AT IN VITRO STUDIES;335 15.3;III. REGENT IN VITRO STUDIES;337 15.4;IV. COMBINED IN VITRO-IN VIVO STUDIES;357 15.5;V. CONCLUSIONS;360 15.6;REFERENCES;363 16;CHAPTER 6. Tissue Culture in Pharmacology;368 16.1;I. INTRODUCTION;368 16.2;II. METHODS OF ASSESSMENT OF DRUG ACTIVITY;372 16.3;III. TISSUE CULTURE IN SCREENING SYSTEMS IN CANCER CHEMOTHERAPY;377 16.4;IV. TISSUE CULTURE IN THE STUDY OF PHARMACOLOGICALLY ACTIVE AGENTS;390 16.5;REFERENCES;405 17;CHAPTER 7. Invertebrate Tissue and Organ Culture in Cell Research;414 17.1;I. INTRODUCTION;414 17.2;II. DESIGN OF CULTURE MEDIA;418 17.3;III. PREPARATION OF MATERIALS AND TECHNIQUES;431 17.4;IV. SOURCES OF CELLS AND TISSUES;435 17.5;V. GROWTH BY CELL DIVISION IN TISSUE CULTURES;437 17.6;VI. AGGREGATION OF DISSOCIATED CELLS;444 17.7;VII. CULTURE OF ORGANS AND EMBRYOS IN DEVELOPMENTAL STUDIES;454 17.8;VIII. INVERTEBRATE TISSUE CULTURE IN PATHOLOGY;463 17.9;REFERENCES;466 18;CHAPTER 8. Introduction and Methods Employed in Plant Tissue Culture;476 18.1;I. INTRODUCTION;476 18.2;II. TECHNIQUES OF ORGAN, TISSUE AND FREE CELL CULTURE;481 18.3;REFERENCES;537 19;CHAPTER 9. The Nutrition and Metabolism of Plant Tissue and Organ Cultures;550 19.1;I. INTRODUCTION;551 19.2;II. ASPECTS OF INORGANIC NUTRITION;554 19.3;III. ASPECTS OF NITROGEN NUTRITION AND METABOLISM;559 19.4;IV. CARBOHYDRATE REQUIREMENTS AND PHYSIOLOGICAL EFFECTS OF SUGARS AND SUGAR ALCOHOLS;571 19.5;V. VITAMIN NUTRITION;579 19.6;VI . REQUIREMENTS FOR GROWTH-
REGULATING SUBSTANCES;586 19.7;VII. PATHWAYS OF BIOSYNTHESIS, "BIOCHEMICAL GENETICS" AND ADAPTATION IN ORGAN AND TISSUE CULTURES;594 19.8;VIII. THE RELEASE OF METABOLITES INTO THE CULTURE MEDIUM;608 19.9;IX. THE RESISTANCE TO GROWTH IN CULTURE ENCOUNTERED WITH SOME ORGANS, TISSUES AND CELLS;616 19.10;REFERENCES;624 20;CHAPTER 10. Growth, Differentiation and Organogenesis in Plant Tissue and Organ Cultures;648 20.1;I. INTRODUCTION;648 20.2;II. GROWTH AND DIFFERENTIATION IN CULTURED ROOTS;649 20.3;III. HlSTOGENESIS IN CALLUS CULTURES;669 20.4;IV. GROWTH AND DIFFERENTIATION IN SUSPENSION CULTURES;682 20.5;V. GENERAL CONCLUSIONS;698 20.6;ACKNOWLEDGEMENTS;698 20.7;REFERENCES;698 21;CHAPTER 11. The Use of Tissue Culture in Phytopathology;708 21.1;I. INTRODUCTION;708 21.2;II. THE CROWN-GALL DISEASE;709 21.3;III. GENETIC TUMOURS;715 21.4;IV. VIRUS TUMOURS;717 21.5;V. SPRUCE TUMOURS;719 21.6;VI. FERN TUMOURS;721 21.7;VII. INSECT GALLS;723 21.8;VIII. NEMATODES;724 21.9;IX. BACTERIAL DISEASES;725 21.10;X. FUNGAL DISEASES;727 21.11;XI. VIRUS DISEASES;729 21.12;ACKNOWLEDGEMENTS;733 22;Author Index;740 23;Taxonomic Index;770 24;Subject Index;774 25;REFERENCES;733


EAN: 9781483277233
Untertitel: 200:Adobe eBook. Sprache: Englisch.
Verlag: Elsevier Science
Erscheinungsdatum: Oktober 2013
Format: pdf eBook
Kopierschutz: Adobe DRM
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