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Cells and Tissues in Culture


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Dezember 2015

Beschreibung

Beschreibung

Cells and Tissues in Culture

Inhaltsverzeichnis

1;Front Cover;1 2;Cells and Tissues in Culture: Methods, Biology and Physiology;6 3;Copyright Page;7 4;Table of Contents ;12 5;Contributors to Volume 1;8 6;Foreword to Volume 1;10 7;Contents of Volume 2;16 8;Contents of Volume 3;17 9;Chapter 1. Introduction;18 9.1;References;32 10;Chapter 2. Methods;36 10.1;I. Basic Requirements;36 10.2;II. Tissue Culture;46 10.3;III. Cell Culture;62 10.4;IV. Organ Culture;87 10.5;References;103 11;Chapter 3. Construction and Use of Synthetic Media;116 11.1;I. Historical Approach;116 11.2;II. Design of Complete Synthetic Media;123 11.3;III. Synthetic Media in Relation to Cells and their Total Environment;138 11.4;IV. Future prospects;147 11.5;References;149 12;Chapter 4. Morphological Problems of Cell Type, Shape and Identification;160 12.1;I. The Problem;160 12.2;II. The Importance of Cell Movement;162 12.3;III. The Main Cell Types;164 12.4;IV. The Significance of the Main Cell Types;165 12.5;V. The Possible Evolution of the Cell Types;176 12.6;VI. Some Physico-chemical Characteristics of the Cell Types;179 12.7;VII. The Problem of Interconversion;181 12.8;VIII. Lessons from the Metaplasia of Naegleria;183 12.9;IX. Cells in Clones and Pure Lines;184 12.10;X. Tissue Culture and the Meaning of Differentiation;189 12.11;References;191 13;Chapter 5. The Locomotory Behaviour of Cells;194 13.1;I. Introduction;194 13.2;II. The Individual Cell;195 13.3;III. Cell Populations;210 13.4;References;215 14;Chapter 6. Cell Division;220 14.1;I. Introduction;220 14.2;II. Mitosis;221 14.3;III. The Cell Cycle;235 14.4;IV. Mitogenesis;244 14.5;V. Synchronism of Divisions in Cultures;246 14.6;VI. Conclusions;247 14.7;References;248 15;Chapter 7. Carbohydrate and Energy Metabolism;256 15.1;I. Introduction;256 15.2;II. Energy Relationships in Growing and Stationary Cells;257 15.3;III. Energy-yielding Reactions;261 15.4;IV. Relationships between Energy Metabolism and Other Functions;271 15.5;V. Influence of Environmental Factors;275 15.6;VI. Stable Differ
ences among Cells;283 15.7;References;285 16;Chapter 8. Amino Acid and Protein Metabolism. I. The Metabolic Characteristics of Serially Propagated Cells;294 16.1;I. Introduction;294 16.2;II. Nutritional Requirements of Serially Propagated Cells;296 16.3;III. Amino Acid and Protein Metabolism in Cell Cultures;304 16.4;IV. General Considerations;310 16.5;References;312 17;Chapter 9. Amino Acid and Protein Metabolism. II. Further Aspects of the Amino Acid and Protein Metabolism of Tissues in Culture;314 17.1;I. Introduction;314 17.2;II. Nutritional Studies;315 17.3;III. Utilization of Amino Acids;319 17.4;IV. Protein Metabolism;322 17.5;V. Conclusion;328 17.6;References;329 18;Chapter 10. Deoxyribonucleic Acid and Ribonucleic Acid Synthesis in Cell Cultures;334 18.1;I. Introduction;334 18.2;II. DNA Synthesis in Cell Cultures;338 18.3;III. RNA Synthesis in Cell Cultures;343 18.4;IV. The Relations between DNA, RNA and Nuclear Protein during the Interphase of Rapidly Dividing Cells;347 18.5;References;361 19;Chapter 11. Mucopolysaccharide Metabolism in Tissue Culture;370 19.1;I. Introduction;370 19.2;II. Analytical and Technical Methods;371 19.3;III. Tissue Culture Studies;382 19.4;IV. Concluding Remarks;402 19.5;References;403 20;Chapter 12. Genetic Cytology;414 20.1;I. Introduction;414 20.2;II. Cytogenetics of Man;416 20.3;III. DNA Synthesis and the Cell Cycle;432 20.4;IV. Heterochromatin and Heteropyknosis;448 20.5;V. Sex Chromatin, Sex Chromosomes, Sex Determination;463 20.6;References;469 21;Chapter 13. Differentiation: Environmental Factors, Chemical and Cellular;480 21.1;I. Introduction;480 21.2;II. Criteria of Differentiation;481 21.3;III. Current Problems of Differentiation in vitro;484 21.4;IV. Cultural Factors and Differentiation;486 21.5;V. Homotypic Factors;488 21.6;VI. Heterotypic Factors;494 21.7;VII. Summary and Prospect;499 21.8;References;501 22;Chapter 14. Recombination of Dissociated Cells and the Development of Cell Aggregates;506 22.1;I. Introduction
;506 22.2;II. Aggregation of Cells;507 22.3;III. The Development of Aggregates;522 22.4;IV. Remarks on the Internal Patterns of Aggregates;536 22.5;References;541 23;Chapter 15. Embryogenesis in vitro;548 23.1;I. Culture of Amphibian Embryonic Anlage;549 23.2;II. In vitro Culture of Embryos of Birds;570 23.3;III. In vitro Culture of Mammalian Ova;592 23.4;IV. Culture in vitro of the Embryos of Fish and Reptiles;599 23.5;References;601 24;Chapter 16. The Action of Hormones on Cell and Organ Culture;608 24.1;I. Introduction;608 24.2;II. Effects of Insulin and Growth Hormone;610 24.3;III. Effects of Thyrotrophin (TSH), Adrenocorticotroph (ACTH) and Gonadotrophins;618 24.4;IV. Action of Corticosteroids and Adrenaline;624 24.5;V. The Effects of Sex Hormones;633 24.6;VI. Influence of Hormones on the Development an Function of the Mouse Mammary Gland;650 24.7;VII. Effects of Thyroxine and Triiodothyronine;656 24.8;VIII. The Effects of Parathyroid Hormone on Bone;662 24.9;IX. Conclusions;666 24.10;References;667 25;Chapter 17. The Effects of Vitamins A and C on Cells and Tissue in Culture;676 25.1;I. Vitamin A;676 25.2;II. Vitamin C;697 25.3;References;708 26;Author Index;718 27;Subject Index;744


EAN: 9781483223001
Untertitel: Methods, Biology and Physiology. 200:Adobe eBook. Sprache: Englisch.
Verlag: Elsevier Science
Erscheinungsdatum: Dezember 2015
Format: pdf eBook
Kopierschutz: Adobe DRM
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