Cells and Tissues in Culture

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Dezember 2015



Cells and Tissues in Culture: Methods, Biology and Physiology, Volume 1 covers the general fields of tissue culture, including an evaluation of its technique, effects, and contributions to biology.

This book focuses on the three methods of culture¿tissue culture, cell culture, and organ culture.Other topics include the design of complete synthetic media, possible evolution of the cell types, and energy relationships in growing and stationary cells. The RNA synthesis in cell cultures, culture of amphibian embryonic anlage, action of corticosteroids and adrenaline, and effects of parathyroid hormone on bone are also elaborated.

This volume is recommended for biologists and specialists interested in the culture of cells and tissues.


1;Front Cover;1 2;Cells and Tissues in Culture: Methods, Biology and Physiology;6 3;Copyright Page;7 4;Table of Contents ;12 5;Contributors to Volume 1;8 6;Foreword to Volume 1;10 7;Contents of Volume 2;16 8;Contents of Volume 3;17 9;Chapter 1. Introduction;18 9.1;References;32 10;Chapter 2. Methods;36 10.1;I. Basic Requirements;36 10.2;II. Tissue Culture;46 10.3;III. Cell Culture;62 10.4;IV. Organ Culture;87 10.5;References;103 11;Chapter 3. Construction and Use of Synthetic Media;116 11.1;I. Historical Approach;116 11.2;II. Design of Complete Synthetic Media;123 11.3;III. Synthetic Media in Relation to Cells and their Total Environment;138 11.4;IV. Future prospects;147 11.5;References;149 12;Chapter 4. Morphological Problems of Cell Type, Shape and Identification;160 12.1;I. The Problem;160 12.2;II. The Importance of Cell Movement;162 12.3;III. The Main Cell Types;164 12.4;IV. The Significance of the Main Cell Types;165 12.5;V. The Possible Evolution of the Cell Types;176 12.6;VI. Some Physico-chemical Characteristics of the Cell Types;179 12.7;VII. The Problem of Interconversion;181 12.8;VIII. Lessons from the Metaplasia of Naegleria;183 12.9;IX. Cells in Clones and Pure Lines;184 12.10;X. Tissue Culture and the Meaning of Differentiation;189 12.11;References;191 13;Chapter 5. The Locomotory Behaviour of Cells;194 13.1;I. Introduction;194 13.2;II. The Individual Cell;195 13.3;III. Cell Populations;210 13.4;References;215 14;Chapter 6. Cell Division;220 14.1;I. Introduction;220 14.2;II. Mitosis;221 14.3;III. The Cell Cycle;235 14.4;IV. Mitogenesis;244 14.5;V. Synchronism of Divisions in Cultures;246 14.6;VI. Conclusions;247 14.7;References;248 15;Chapter 7. Carbohydrate and Energy Metabolism;256 15.1;I. Introduction;256 15.2;II. Energy Relationships in Growing and Stationary Cells;257 15.3;III. Energy-yielding Reactions;261 15.4;IV. Relationships between Energy Metabolism and Other Functions;271 15.5;V. Influence of Environmental Factors;275 15.6;VI. Stable Differ
ences among Cells;283 15.7;References;285 16;Chapter 8. Amino Acid and Protein Metabolism. I. The Metabolic Characteristics of Serially Propagated Cells;294 16.1;I. Introduction;294 16.2;II. Nutritional Requirements of Serially Propagated Cells;296 16.3;III. Amino Acid and Protein Metabolism in Cell Cultures;304 16.4;IV. General Considerations;310 16.5;References;312 17;Chapter 9. Amino Acid and Protein Metabolism. II. Further Aspects of the Amino Acid and Protein Metabolism of Tissues in Culture;314 17.1;I. Introduction;314 17.2;II. Nutritional Studies;315 17.3;III. Utilization of Amino Acids;319 17.4;IV. Protein Metabolism;322 17.5;V. Conclusion;328 17.6;References;329 18;Chapter 10. Deoxyribonucleic Acid and Ribonucleic Acid Synthesis in Cell Cultures;334 18.1;I. Introduction;334 18.2;II. DNA Synthesis in Cell Cultures;338 18.3;III. RNA Synthesis in Cell Cultures;343 18.4;IV. The Relations between DNA, RNA and Nuclear Protein during the Interphase of Rapidly Dividing Cells;347 18.5;References;361 19;Chapter 11. Mucopolysaccharide Metabolism in Tissue Culture;370 19.1;I. Introduction;370 19.2;II. Analytical and Technical Methods;371 19.3;III. Tissue Culture Studies;382 19.4;IV. Concluding Remarks;402 19.5;References;403 20;Chapter 12. Genetic Cytology;414 20.1;I. Introduction;414 20.2;II. Cytogenetics of Man;416 20.3;III. DNA Synthesis and the Cell Cycle;432 20.4;IV. Heterochromatin and Heteropyknosis;448 20.5;V. Sex Chromatin, Sex Chromosomes, Sex Determination;463 20.6;References;469 21;Chapter 13. Differentiation: Environmental Factors, Chemical and Cellular;480 21.1;I. Introduction;480 21.2;II. Criteria of Differentiation;481 21.3;III. Current Problems of Differentiation in vitro;484 21.4;IV. Cultural Factors and Differentiation;486 21.5;V. Homotypic Factors;488 21.6;VI. Heterotypic Factors;494 21.7;VII. Summary and Prospect;499 21.8;References;501 22;Chapter 14. Recombination of Dissociated Cells and the Development of Cell Aggregates;506 22.1;I. Introduction
;506 22.2;II. Aggregation of Cells;507 22.3;III. The Development of Aggregates;522 22.4;IV. Remarks on the Internal Patterns of Aggregates;536 22.5;References;541 23;Chapter 15. Embryogenesis in vitro;548 23.1;I. Culture of Amphibian Embryonic Anlage;549 23.2;II. In vitro Culture of Embryos of Birds;570 23.3;III. In vitro Culture of Mammalian Ova;592 23.4;IV. Culture in vitro of the Embryos of Fish and Reptiles;599 23.5;References;601 24;Chapter 16. The Action of Hormones on Cell and Organ Culture;608 24.1;I. Introduction;608 24.2;II. Effects of Insulin and Growth Hormone;610 24.3;III. Effects of Thyrotrophin (TSH), Adrenocorticotroph (ACTH) and Gonadotrophins;618 24.4;IV. Action of Corticosteroids and Adrenaline;624 24.5;V. The Effects of Sex Hormones;633 24.6;VI. Influence of Hormones on the Development an Function of the Mouse Mammary Gland;650 24.7;VII. Effects of Thyroxine and Triiodothyronine;656 24.8;VIII. The Effects of Parathyroid Hormone on Bone;662 24.9;IX. Conclusions;666 24.10;References;667 25;Chapter 17. The Effects of Vitamins A and C on Cells and Tissue in Culture;676 25.1;I. Vitamin A;676 25.2;II. Vitamin C;697 25.3;References;708 26;Author Index;718 27;Subject Index;744

EAN: 9781483223001
Untertitel: Methods, Biology and Physiology. 200:Adobe eBook. Sprache: Englisch. Dateigröße in MByte: 135.
Verlag: Elsevier Science
Erscheinungsdatum: Dezember 2015
Seitenanzahl: 804 Seiten
Format: pdf eBook
Kopierschutz: Adobe DRM
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