Amino Acid Analysis Protocols

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September 2000



Amino acid analysis is a technique that has become commonplace in biotechnology, biomedical, and food analysis laboratories. This book describes a variety of amino acid analysis techniques and how each technique can be used to answer specific biological questions. The first two chapters in Amino Acid Analysis Protocols introduce the concepts, basic theory, and practice of amino acid analysis. The following chapters give detailed instructions on various methods and their applications. As highlighted, there are many different approaches to amino acid ana- sis, but in all cases the results depend heavily on the quality of the sample. Therefore a new way to desalt samples prior to hydrolysis is covered as an introductory chapter (Chapter 3), and most authors have devoted a section to sample preparation, especially to the collection and storage of bodily fluids. Some of the amino acid analysis methods described in this book are based on HPLC separation and analysis after precolumn derivatization. The precolumn derivatization techniques described use (a) 6-aminoquinolyl-N-hydro- succinimidyl carbamate (AQC) (Chapters 4 and 8); (b) 1-fluoro-2- dinitrophenyl-5-L-alanine amide (Marfey's reagent), which allows sepa- tion and analysis of enantiomeric amino acids (Chapter 5); (c) O-phthalal- hyde (OPA) (Chapters 6 and 10); (d) butylisothiocyanate (BITC) and benzylisothiocyante (BZITC) (Chapter 11); (e) phenylisothiocyanate (PITC) (Chapters 12 and 13); (f) ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-s- fonate (SBD-F) (Chapter 17); and (g) 9-fluorenylmethyl-chloroformate (FMOC-Cl) (Chapter 10).


Amino Acid Analysis: An Overview, Margaret I. Tyler. Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory, Frank D. Macchi, Felicity J. Shen, Rodney G. Keck, and Reed J. Harris. Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Analysis, Li Zhang and Nancy Denslow. Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate Steven A. Cohen. Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-l-Alanine Amide (Marfey's Reagent), Sunil Kochhar, Barbara Mouratou, and Philipp Christen. The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection, C. David Forster and Charles A. Marsden. Anion Exchange Chromatography and Intergrated Amperometric Detection of Amino Acids, Petr Jandik, Christopher Pohl, Victor Barreto, and Nebojsa Avdalovic. Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane, Noriko Shindo, Tsutomu Fujimura, Saiko Kazuno, and Kimie Murayama. Capillary Gas Chromatographic Analysis of Protein and Nonprotein Amino Acids in Biological Samples, Hiroyuki Kataoka, Sayuri Matsumura, Shigeo Yamamoto, and Masami Makita. Measurement of Blood Plasma Amino Acids in Ultrafiltrates by High-Performance Liquid Chromatography with Automatic Precolumn O-Phthaldialdehyde Derivatization, Hua Liu. Determination of Amino Acids in Foods by Reversed-Phase High-Performance Liquid Chromatography with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared to the Phenylthiocarbamyl Derivative and Ion Exchange Chromatography, Kang-Lyung Woo. Amino Acid Measurement in Body Fluids Using PITC Derivatives, Roy A. Sherwood. Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials by Analysis. of Phenylthiocarbamyl Derivatives, Margareta Stark and Jan Johansson. Analysis of O-Phosphoamino Acids in Biological Samples by Gas Chromatography with Flame Photometric Detection, Hiroyuki Kataoka, Norihisa Sakiyama, Yukizo Ueno, Kiyohiko Nakai, and Masami Makita. Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection, Hiroyuki Kataoka, Kiyomi Takagi, Hirofumi Tanaka, and Masami Makita. Capillary Electrophoretic Determination of 4-Hydroxyproline, Qingyi Chu and Michael Zeece. Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization, Isabella Fermo and Rita Paroni. Determination of Early Glycation Products by Mass Spectrometry and Quantification of Glycation Mediated Protein Crosslinks by the Incorporation of [14C]lysine into Proteins, Malladi Prabhakaram, Beryl J. Ortwerth, and Jean B. Smith. Index.


"Sufficient detail and reference material is provided to allow application by a chemist generally familiar with analytical methods. Choices among the various procedures described will likely be made on an empirical basis."-Doody's Health Sciences Book Review Journal
"...the book presents a timely and detailed methodological reference for a broad range of current techniques used in amino acid analysis. The authors are all experts in the field and the editors (especially Williams) have considerable experience in amino acid analysis. this volume will provide an indispensable tool in any laboratory carrying out amino acid analysis."- Today's Life Science
"...This is a major collection of analytical techniques devoted to the analysis of amino acids in biotechnology, food analysis, and biomedical research, as well as the amino acid content of patient samples in clinical laboratories...This book is highly recommended for both engineers and experienced analysts interested in amino acid analysis; both will benefit from the information contained in this collection. It might also be a helpful resource for those looking for a replacement for old, dedicated amino acid analyzers for physiological samples by methods based on newer technologies and alternative techniques." - Clinical Chemistry
"Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists as well as a broad range of biological and biomedical investigators a richer compendium of laboratory tools for the productive analysis of both common and uncommon amino acids." - Journal of Chromatographic Science
EAN: 9780896036567
ISBN: 0896036561
Untertitel: 'Methods in Molecular Biology'. 2000. Auflage. Sprache: Englisch.
Erscheinungsdatum: September 2000
Seitenanzahl: 265 Seiten
Format: gebunden
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