HUDU

Phospholipid Signaling Protocols


€ 162,99
 
gebunden
Lieferbar innert 2 Wochen
April 1998

Beschreibung

Beschreibung

Cell membranes are not, as once believed, inert structures designed to contain the cell contents, but are in fact dynamic structures that are as me- bolically active as the cytosol and other cellular compartments they surround. Thus membranes not only contain mixtures of lipid and phospholipids, but also many proteins both embedded deeply within the membrane structure itself and also more loosely attached on the membrane surfaces. Though many such proteins have long been known to act as transport proteins, ion channels, hormone receptors, G proteins, cytoskeletal anchorage points, and so on, the major advance of recent years is the increasing understanding that the lipids and phospholipids in the membrane bilayer itself are also metabolized to b- logically active products that can diffuse either in the cytosol or in the m- brane bilayer to control the function of other proteins. Thus the concept of lipid-derived second messengers is now firmly established.

Inhaltsverzeichnis

Part I. Monitoring of Activation of Phospholipid-Derived Cell Signaling Pathways

Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H] Phosphoinositides and [3H] Phosphoinositols
Ian M. Bird

Phosphoinositidase C Activation Assay II: Simple Analysis of Recovered Cellular Phosphoinositides and Phosphoinositols
Ian M. Bird

Phosphoinositidase C Activation Assay III: HPLC Analysis of Cellular Phosphoinositides and Phosphoinositols
Ian M. Bird

Measurement of Phosphoinositols and Phosphoinositides Using Radio High-Performance Liquid Chromatography Flow Detection
Lubo Zhang and Iain L. O. Buxton

Preparation of [3H]Phosphoinositol Standards and Conversion of [3H] Phosphoinositides to [3H] Phosphoinositols
Ian M. Bird

Inositol 1,4,5-Trisphosphate Mass Assay
Lubo Zhang

Measurement of Cellular Diacylglycerol Content
Wendy Bollinger Bollag and Richard D. Griner

Phosphatidylinositol 4-Kinases: Assays and Product Analysis
Rachel E. Meyers and Lewis C. Cantley

Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Displacement Assay
Jeroen van der Kaay, Pete J. Cullen, and C. Peter Downes

Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography
Gregory J. Parker, Joost C. Loijens, and Richard A. Anderson

Determination of Phospholipase C- or Phospholipase D-Catalyzed Phosphatidylcholine Hydrolysis
Michael J. O. Wakelam and Trevor R. Pettitt

Measurement of Phospholipase D Activity
Wendy Bollinger Bollag

Monitoring of Phospholipase A2 Activation in Cultured Cells Using Tritiated Arachidonic Acid
Sandie I. Briand, Sylvie G. Bernier, and Gaétan Guillemette

Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases
Bryan A.Wolf

Isotopic Efflux Studies as Indices of Phospholipase Activation
Jacob Vadakekalam and Stewart Metz

Extraction and Measurements of Prostanoids and Leukotrienes by Radioimmunoassays
Bruno Battistini, Serge Picard, Pierre Borgeat, and Pierre Sirois

Measurement of Prostanoids, Leukotrienes, and Isoprostanes By Enzyme Immunoassays
Bruno Battistini, Serge Picard, Pierre Borgeat, and Pierre Sirois

Measurements of Arachidonic Acid Metabolites Derived from the Lipoxygenase Pathways By High-Pressure Liquid Chromatography
Pierre Borgeat, Serge Picard, Bruno Battistini, and Pierre Sirois

Measurement of Sphingomyelin and Ceramide Cellular Levels After Sphingomyelinase-Mediated Sphingomyelin Hydrolysis
Pino Santana, Luisa F. Fanjul, and C. M. Ruiz de Galarreta

Sphingomyelin and Ceramide Mass Assay
Pino Santana, C. M. Ruiz de Galarreta, and Luisa F. Fanjul

Sphingosine Kinase: Assay and Product Analysis
Ana Olivera and Sarah Spiegel

Analytical Methods and Steps to Sample Preparation For Determination of Molecular Species of Fatty Acids
Sanda Clejan

HPLC Analytical Methods for the Separation of Molecular Species of Fatty Acids In Diacylglycerol and Cellular Phospholipids
Sanda Clejan

Analysis of Molecular Species of Cellular Sphingomyelins and Ceramides
Sanda Clejan

Part II. General methods for monitoring changes in levels of key signaling pathway proteins and associated mRNA

Solubilization and Assay of Cellular and Tissue Protein
Cynthia E. Shaw

Western Immunoblot Analysis
Cynthia E. Shaw and Jing Zheng

Immunohistochemistry and Immunocytochemistry
Jing Zheng

Extraction of Cellular and Tissue RNA
Daniel S. Millican and Ian M. Bird

Size Separation and Quantification of mRNA by Northern Analysis
Ian M. Bird

Preparation of

Pressestimmen

". . . the detailed information provided in Phospholipid Signaling Protocols makes it a welcome addition to an academic library that serves scientists actively working in the area of second messenger and membrane lipid chemistry. It is well representative of the previous volumes in the Methods in Molecular Biology series, and therefore is a worthwhile acquisition."-E-Streams
". . .this book is an extremely valuable resource for methodology in studying the molecular species of key phospholipid signaling molecules, as well as the enzymes that produce or degrade them. I personally have used several protocols in this book, and have not had any trouble adapting the methods to my own applications."-The Quarterly Review of Biology
"...gives an excellent overview of the most common techniques in use as well in biochemistry and molecular biology...The editor is to be congratulated for putting these diverse, very important protocols together..."-Cell Biology International 2000
"...does a very good job of covering what is a fairly diverse field and the protocols described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signaling molecules."-Molecular Biotechnology
"...does a very good job covering what is a fairly diverse field and the protocols described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signalling molecules." - Molecular Biotechnology
EAN: 9780896034914
ISBN: 0896034917
Untertitel: 1998. Auflage. Sprache: Englisch.
Verlag: SPRINGER VERLAG GMBH
Erscheinungsdatum: April 1998
Seitenanzahl: 380 Seiten
Format: gebunden
Es gibt zu diesem Artikel noch keine Bewertungen.Kundenbewertung schreiben