Human Gametes and Preimplantation Embryos

€ 180,49
Sofort lieferbar
Juni 2013



In recent years, the advancing science and increasing availability of assisted reproduction have given new hope to infertile couples. However, the use of IVF and ART has also led to marked increases in the number of multiple-infant live births. This poses a public health concern, as these neonates have a higher rate of pre-term delivery, compromising their survival chances and increasing their risk of lifelong disability. By optimizing the selection of gametes and embryos with high probabilities of implantation, it is possible to reduce the number of embryos transferred and, by extension, the number of high-risk multiple gestations, while maintaining or increasing pregnancy rates. Human Gametes and Preimplantation Embryos: Assessment and Diagnosis provides a broad yet concise overview of established and developing methodologies for assessment of gamete and embryo viability in assisted reproduction. This book elucidates the best practices for precisely selecting viable specimens based on morphology and cleavage rate and covers the spectrum of emerging adjunctive technologies for predicting reproductive potential. The authors present their extensive knowledge of "omics" approaches (genomics, transcriptomics, proteomics, and metabolomics), with unbiased delineation of the associated advantages and potential pitfalls. This valuable clinical resource is well suited to infertility specialists, Ob/Gyn physicians, IVF laboratory technicians, and researchers in the fields of embryology and reproductive medicine.


1. Current Practices and Regulations for Embryo Transfer Worldwide: Implications on IVF Outcome
Baris Ata and Emre Seli
2. Analysis of the Zona Pellucida as an Indicator of Oocyte Developmental Potential
Markus Montag, Thomas Schimming, and Maria Köster
3. Morphological Assessment of Oocytes, Pronuclear and Cleavage Stage Embryos
Laura Rienzi, Benedetta Iussig, and Filippo Ubaldi
4. Morphological Assessment of Blastocyst Stage Embryos: Types of Grading Systems and Their Reported Outcomes
Basak Balaban and David K. Gardner
5. Real Time Imaging Strategies to Improve Morphological Assessment
María Cruz, Manuel Muñoz, and Marcos Meseguer
6. Limitations and Benefits of Morphologic Embryo Assessment Strategies: How Far Can Morphological Assessment Go in the Identification of Viable Embryos?
Denny Sakkas and David K. Gardner
7. Sperm Morphologic Characteristics and Their Impact on Embryo Quality and Pregnancy Outcome
Bhushan K. Gangrade and Ashok Agarwal
8. Objective Biomarkers of Sperm Development and Fertility: Assessment of Sperm-Zona Pellucida Binding Ability and Hyaluronic Acid Mediated Selection of Sperm for ICSI Fertilization
Gabor Huszar
9. Selecting the Best Sperm and Its Implications in Clinical Practice
Juan G. Alvarez
10. The Origins of Aneuploidy in Human Embryos
Elpida Fragouli and Joy Delhanty
11. Preimplantation Genetic Screening and Diagnosis Using Fluorescent In Situ Hybridization (FISH)
Tsilya Gerasimova and Maria D. Lalioti
12. Single Nucleotide Polymorphisms and Next Generation Sequencing
Alan H. Handyside and Dagan Wells
13. Use of Comparative Genomic Hybridization (CGH) and Microarray-CGH for Preimplantation Genetic Screening
Leeanda Wilton and Dagan Wells
14. Quantitative SNP Array and Real-Time PCR Based Human Preimplantation Embryo Aneuploidy Screening
Nathan R. Treff
15. Regulation of Gene Expression in the Oocyte and Early Embryo: Implications for Transcriptomic and Proteomic Embryo Assessment
Helena Kristiansson and Emre Seli
16. Transcriptomics Technology: Promise and Potential Pitfalls
Can Bruce and Asli Uyar
17. Transcriptomic Analysis of Cumulus and Granulosa Cells as a Marker of Embryo Viability
Elpida Fragouli  and Dagan Wells
18. Mass Spectrometry, Proteomics and the Study of Sperm Cell Biology
Mark A. Baker and R. John Aitken
19. Proteomic Analysis of Embryo Viability
Mandy G Katz-Jaffe and Susanna McReynolds
20. Metabolism of the Viable Human Embryo
David K. Gardner
21. Analysis of Respiration as an Indicator of Oocyte and Embryo Developmental Potential
David L. Keefe
22. Mitochondrial Activity as a Biomarker of Gamete and Embryo Health
Jonathan Van Blerkom
23. Carbohydrate Analysis and Embryo Viability
Michelle Lane and David K. Gardner
24. Identification of Viable Embryos by Non-invasive Measurement of Amino Acids in Culture Media
Franchesca D. Houghton
25. Metabolomic Profiling of Embryos Using Spectroscopy
Denny Sakkas and Emre Seli
26. Microfluidic Devices for the Analysis of Gamete and Embryo Physiology
George A. Thouas, David L. Potter, and David K. Gardner

EAN: 9781461466505
ISBN: 1461466504
Untertitel: Assessment and Diagnosis. 2013. Auflage. 16 schwarz-weiße und 48 farbige Abbildungen, 8 schwarz-weiße Tabellen. Book. Sprache: Englisch.
Verlag: Springer-Verlag GmbH
Erscheinungsdatum: Juni 2013
Seitenanzahl: XV
Format: gebunden
Es gibt zu diesem Artikel noch keine Bewertungen.Kundenbewertung schreiben