Helicobacter Pylori Protocols

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März 1997



Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.


Introduction: Medical Significance of H. pylori
Martin J. Blaser

Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture
Stewart Goodwin

Serology and Urea Breath Test in the Diagnosis of H. pylori Infection
Peter R. Hawtin

The Detection of H. pylori by the Polymerase Chain Reaction
Nicholas P. Mapstone

PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples
Stephen B. Fritz and T. Ulf Westblom

In Vitro Susceptibility Testing of H. pylori, Alistair McLaren. Defined Media for H. pylori
David J. Reynolds

Studies of Respiration in H. pylori
Roger J. Miles and Huai-Tzu Chang

Use of NMR to Study H. pylori Metabolism
Peter A. Chalk, Andrew D. Roberts, Alistair A. Davison, David J. Kelly, and Peter J. White

Isolation of H. pylori Genomic DNA and Restriction Analysis
Robert J. Owen and Jane Bickley

Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori
Wee Tee

Differentiation of H. pylori Strains Using PCR RFLP
Harry L. T. Mobley and Paul A. Foxall

PCR-DNA Sequence Typing
Agnès Labigne

H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method
Douglas E. Berg, Janaki Lelwala-Guruge, Engin T. Incecik, Kalpana Srivastava, and Natalia S. Akopyants

Molecular Methods for Detecting Ulcerogenic Strains of H. pylori
John C. Atherton

H. pylori DNA Transformation by Natural Competence and Electroporation
Zhongming Ge and Diane E. Taylor

Random Mutagenesis of the H. pylori Genome
Agnès Labigne

Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE)
Nicholas Chang, Qin Jiang, and Diane E. Taylor

Molecular Characterization of H. pylori SurfaceAntigens
Peter Doig, Paul W. O'Toole, and Trevor J. Trust

Isolation and Characterization of Porins from H. pylori
Robert E. W. Hancock and Maurice Exner

Methods for the Identification of H. pylori Host Receptors
Thomas Borén, Torkel Wadström, Staffan Normark, Jeffrey I. Gordon, and Per G. Falk

Host Responses to H. pylori: Molecular Analysis of Cytokine Gene Expression
Susan M. Farmery and Jean E. Crabtree

Nonprimate Animal Models of H. pylori Infection
Andrew A. McColm

The Nonhuman Primate Model for H. pylori Infection
Andre Dubois and Douglas E. Berg



"The book was designed to provide up-to-date detailed protocols for the experienced either working
in the field of Helicobacter research or wishing to enter the field. The authors share their experience and give advice about how to avoid pitfalls and problems encountered with this organism. The book is recommended for those with a serious interest in microbiology and molecular biology of Helicobacter pylori. - David Graham, MD, Baylor College of Medicine
EAN: 9780896033818
ISBN: 0896033813
Untertitel: 'Methods in Molecular Medicine'. 1997. Auflage. Sprache: Englisch.
Erscheinungsdatum: März 1997
Seitenanzahl: 274 Seiten
Format: gebunden
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