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Haemophilus Influenzae Protocols


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September 2002

Beschreibung

Beschreibung

As the second leading bacterial cause of community-acquired pneumonia, H. influenzae accounts for many thousands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of this significant bacterium. Described with step-by-step instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. The antibody techniques presented include IVIAT, ELISA, and opsonophagocytosis assays. Also included are reviews of the pathogenesis of NTHi and Hib, methods for working with the organism, culture and storage conditions, molecular diagnosis, and systems for exploring pathogenesis in both in vitro and animal models. Comprehensive and highly practical, Haemophilus influenzae Protocols offers a unique collection of powerful molecular methods that illuminate not only how the bacterium causes disease, but also how best to develop much-needed new vaccines and antibiotics against it.

Inhaltsverzeichnis

The Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae
Gail G. Hardy, Simone M. Tudor, and Joseph W. St. Geme, III.

The Pathogenesis of Disease Due to Type b Haemophilus influenzae
Ruth Aubrey and Christoph Tang

General Methods for Culturing Haemophilus influenzae
Grant Poje and Rosemary J. Redfield

Transformation of Haemophilus influenzae
Grant Poje and Rosemary J. Redfield

Diagnosis of Infection
Alex van Belkum and Loek van Alphen

Characterization of Plasmids
Ioanna D. Dimopoulou, Mohd-Zain Zaini, and Derrick W. Crook

Protein Characterization by Two-Dimensional Gel Electrophoresis
Phillip Cash and J. Simon Kroll

Monitoring Gene Expression Using DNA Arrays
Tahir R. Ali, Ming-Shi Li, and Paul R. Langford

Gene Expression Technology
Xavier de Bolle and Christopher D. Bayliss

The Genome Sequence of Haemophilus influenzae
Derek W. Hood

Structural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzae
Elke K. H. Schweda and James C. Richards

Mutagenesis of H. influenzae
Mark A. Herbert

Transposon Tn10
Stefan Schlör, Gabriele Kemmer, and Joachim Reidl

In Vivo Expression of Bacterial Genes During Human Infections
Martin Handfield, Trevor Seifert, and Jeffrey D. Hillman

ELISA
Joyce S. Plested, Philip A. Coull, and Margaret Anne J. Gidney

Opsonophagocytosis Assay Using Flow-Cytometry
Joyce S. Plested and Philip A. Coull

In Vitro Models of Infection I-Human Respiratory Tissue Organ Culture
Andrew M. Middleton, Paula Keig, and Robert Wilson

n Vitro Models of Infection II-Human Umbilical Vein Endothelial Cells (HUVECs) System
Mumtaz Virji and Darryl J. Hill

Animal Models

Index

Pressestimmen

"This excellent book is recommended for investigators involved in laboratory research on H. influenzae from the beginning student to the experienced investigator. The book contains abundant, detailed protocols and practical tips that will be useful for all investigators." - Clinical Infectious Diseases
"...an excellent source book and an inspiring reading." - International Journal of Medical Microbiology
"In addition to detailed information provided in the individual chapters, the book is also valuable by providing an extensive list of 841 references....there are not many microbiological books of such high quality. The authors present highly specialized information in a comprehensible and friendly way." -Folia Microbioligica
EAN: 9780896039285
ISBN: 0896039285
Untertitel: 2003. Auflage. Sprache: Englisch.
Verlag: SPRINGER VERLAG GMBH
Erscheinungsdatum: September 2002
Seitenanzahl: 333 Seiten
Format: gebunden
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