HUDU

Chemical Proteomics


€ 141,49
 
gebunden
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November 2011

Beschreibung

Beschreibung

The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function.  In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.  Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.

Inhaltsverzeichnis

Part I: Introduction 1. Mass Spectrometry-Based Chemoproteomic Approaches Marcus Bantscheff 2. Chemical Proteomics in Drug Discovery Gerard Drewes Part II: Small Molecules and Probe Design 3. Compound Immobilization and Drug-Affinity Chromatography Uwe Rix, Manuela Gridling, and Giulio Superti-Furga 4. Affinity-based Chemoproteomics with Small Molecule-peptide Conjugates Chaitanya Saxena 5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine Xia Ge and Daniel S. Sem 6. Probing Proteomes with Benzophenone Photoprobes Akira Kawamura and Doina M. Mihai 7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler 8. Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS) Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger Part III: Target Discovery and Target Validation 9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr 10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff 11. Affinity-Based Profiling of Dehydrogenase Subproteomes Xia Ge and Daniel S. Sem 12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten 13. Fluorescence-Based Proteasome Activity Profiling Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers 14. Chemical Cross-Linking and High Resolution Mass Spectrometry to Study Protein-Drug Interactions Mathias Q. Müller and Andrea Sinz 15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry Natalia Gasilova, Alexis Nazabal 16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies Amy Hye Won Jeon, Gerold Schmitt-Ulms Part IV: Ligand Discovery 17. Ligand Discovery Using Small-molecule Microarrays Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler 18. Working with Small-Molecules-Preparing and Storing Stock Solutions and Determination of Kinetic Solubility Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard 19. A Database for Chemical Proteomics-ChEBI Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck 20. Working with Small Molecules: Rules-of-Thumb of "Drug Likeness" Ming-Qiang Zhang
EAN: 9781617793639
ISBN: 1617793639
Untertitel: Methods and Protocols. 'Methods in Molecular Biology'. 2012. Auflage. Book. Sprache: Englisch.
Verlag: Humana Press
Erscheinungsdatum: November 2011
Seitenanzahl: 328 Seiten
Format: gebunden
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