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April 2015



This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.  Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.


1. Generating Primary Cultures of Murine Cardiac Myocytes and Cardiac Fibroblasts to Study Viral Myocarditis
            Barbara Sherry
2. Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts
            Sreejit Parameswaran, Rajalakshmi Santhakumar, Prasanna Vidyasekar, and Rama S. Verma
3. Deep Sequencing of Cardiac MicroRNA-mRNA Interactomes in Clinical and Experimental Cardiomyopathy
            Scot J. Matkovich and Gerald W. Dorn, II
4. Next Generation Sequencing Technology in the Genetics of Cardiovascular Disease
            Cecilia Vecoli
5. Computational Cardiac Electrophysiology: Implementing Mathematical Models of Cardiomyocytes to Simulate Action Potentials of the Heart
            Michael M. Bell and Elizabeth M. Cherry
6. Methods of Myofibrillogenesis Modeling
            Nancy K. Drew and Anna Grosberg
7. Using the Mechanical Bidomain Model to Analyze the Biomechanical Behavior of Cardiomyocytes
            Bradley J. Roth
8. Fabrication of a Myocardial Patch with Cells Differentiated from Human Induced-Pluripotent Stem Cells
            Lei Ye, Joydeep Basu, and Jianyi Zhang
9. Efficient Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells with Growth Factors
            Rajneesh Jha, Ren-He Xu, and Chunhui Xu
10. Isolation, Culturing, and Characterization of Cardiac Muscle Cells from Non-Human Primate Heart Tissue
            Steven M. Hoynowski and John W. Ludlow
11. Mouse Embryonic Stem Cell-Derived Cardiac Myocytes in a Cell Culture Dish
            Carley Glass, Reetu Singla, Anshu Arora, and Dinender K. Singla
12. Cryopreservation of Neonatal Cardiomyocytes
            Adam C. Vandergriff, M. Taylor Hensley, and Ke Cheng
13. Evaluation of Sarcomeric Organization in Human Pluripotent Stem Cell-Derived Cardiomyocytes
            Chrishan J.A. Ramachandra and Winston Shim
14. Electrotonic Coupled Metabolic Purification of Chick Cardiomyocytes
            Winston Shim, Haiyang Yu, K.P. Myu Mai Ja, Muhammad Parasuram, Kee Pah Lim, and Philip Wong
15. Gene Transfer into Cardiac Myocytes
            Sarah E. Lang and Margaret V. Westfall
16. Analysis of 4D Myocardial Wall Motion During Early Stages of Chick Heart Development
            Madeline Midgett and Sandra Rugonyi


EAN: 9781493925711
ISBN: 1493925717
Untertitel: Methods and Protocols. 'Methods in Molecular Biology'. 11 schwarz-weiße und 22 farbige Abbildungen, 5 schwarz-weiße Tabellen, Bibliographie. Book. Sprache: Englisch.
Verlag: Springer-Verlag GmbH
Erscheinungsdatum: April 2015
Seitenanzahl: 216 Seiten
Format: gebunden
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