Biological Low-Voltage Scanning Electron Microscopy

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Januar 2008



Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapter s in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.


Table of Contents
1. Low Voltage Scanning Electron Microscopy In Biology--Historical Preface :
The Early Development of the Scanning Electron Microscope to 1965...1
Dennis McMullan

2. LVSEM For Biology...27
James B. Pawley, PhD

3. The Aberration-Corrected SEM...101
David Joy Science and Engineering Research Facility, University of Tennessee

4. Noise and its effects on the Low Voltage SEM...121
David C Joy

5. High-Resolution Low Voltage Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols...146
Heide Schatten, PhD
6. Molecular labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM...173
Ralph Albrecht and Daryl Meyer

7. Low kV and video-rate, beam-tilt stereo for viewing live-time experiments in the SEM...209
Alan Boyde

8. CryoSEM of Chemically Fixed Animal Cells...228
Stanley L. Erlandsen
9. High resolution and low voltage SEM of plant cells...249
Guy Cox, Peter Vesk, Teresa Dibbayawan, Tobias I. Baskin and Maret Vesk

10. High Resolution Cryo-Scanning Electron Microscopy of Biological Samples...268
Paul Walther

11. Developments in Instrumentation for Microanalysis in Low Voltage Scanning Electron Microscopy...305
Dale E. Newbury


From the reviews:
"Topics ranging from the early development of the scanning electron microscope (SEM) to some of the latest low-voltage and cryo techniques are reviewed in detail. ... this is a very useful volume for those wishing to learn, or refresh their memory, in the context of getting the best out of an SEM, and in particular the field emission SEM (FESEM)." (Iolo Ap Gwynn, infocus Magazine, Issue 12, December, 2008)
"Biological Low-voltage Scanning Electron Microscopy is an excellent and timely collection, written by many of the recognized experts in the subject ... . Strongly recommended." (Ultramicroscopy, Vol. 109, June, 2009)
EAN: 9780387729701
ISBN: 0387729704
Untertitel: Anniversary. 13 farbige Abbildungen, 13 farbige Fotos. Sprache: Englisch.
Verlag: Springer-Verlag GmbH
Erscheinungsdatum: Januar 2008
Seitenanzahl: X
Format: gebunden
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