Bacterial Toxins: Methods and Protocols

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März 2000



The interest of investigators across a broad spectrum of scientific dis- plines has been steadily stimulated by the field of bacterial toxin research, an area that makes use of a large variety of biological, chemical, physicochemical, and medically oriented approaches. Researchers studying bacterial toxins need to be acquainted with all these disciplines in order to work effectively in the field. To date, there has been no published collection offering detailed descr- tions of the techniques and methods needed by researchers operating across the field'sdiverse areas. The present volume Bacterial Toxins: Methods and Pro- cols, is intended to fill this gap. Bacterial Toxins: Methods and Protocols consists of two sections: one on protein toxins (15 chapters) and one on endotoxins (5 chapters). Each s- tion is introduced by an overview article (Chapters 1 and 16). The protocols collected represent state-of-the-art techniques that each have high impact on future bacterial toxin research. All methods are described by authors who have regularly been using the protocol in their own laboratories. Included in each chapter is a brief introduction to the method being described.


Bacterial Protein Toxins: An Overview, Joseph E. Alouf. Purification of Clostridium botulinum Type A Neurotoxin, Carl J. Malizio, Michael C. Goodnough, and Eric A. Johnson. Shiga Toxins, David W. K. Acheson, Anne V. Kane, and Gerald T. Keusch. Isolation and Detection of Microcystins and Nodularins, Cyanobacterial Peptide Hepatotoxins, Jussi Meriluoto, Linda Lawton, and Ken-ichi Harada. Genetic Construction, Expression, and Characterization of Diphtheria Toxin-Based Growth Factor Fusion Proteins, Johanna C. vanderSpek and John R. Murphy. Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins, Jean-Louis Schwartz and Luke Masson. Use of Fourier-Transformed Infrared Spectroscopy for Secondary Structure Determination of Staphylococcal Pore-Forming Toxins, Gianfranco Menestrina. The Use of Fluorescence Resonance Energy Transfer to Detect Conformational Changes in Protein Toxins, William D. Picking. Site-Directed Spin Labeling of Proteins: Applications to Diphtheria Toxin, Kyoung Joon Oh, Christian Altenbach, R. John Collier, and Wayne L. Hubbell. Characterization of Molecular Properties of Pore-Forming Toxins with Planar Lipid Bilayers, Mauro Dalla Serra and Gianfranco Menestrina. Determination of Affinity and Kinetic Rate Constants Using Surface Plasmon Resonance, Luke Masson, Alberto Mazza, and Gregory De Crescenzo. ADP-Ribosylation of a-Gi Proteins by Pertussis Toxin: Positional Dissection of Acceptor Sites Using Membrane Anchored Peptides, Lars von Olleschik-Elbheim, Ali el Bayâ, and M. Alexander Schmidt. Phage Libraries for Generation of Anti-Botulinum scFv Antibodies, Peter Amersdorfer and James D. Marks. T-Cell Cytotoxicity Assays for Studying the Functional Interaction Between the Superantigen Staphylococcal Enterotoxin A and T-Cell Receptors, Alexander Rosendahl, Karin Kristensson, Kristian Riesbeck, and Mikael Dohlsten. In Vitro PhysiologicalStudies on Clostridial Neurotoxins: Biological Models and Procedures for Extracellularand Intracellular Application of Toxins, Bernard Poulain, Marie-France Bader, and Jordi Molgó. The Biology of Endotoxin, Volker T. El-Samalouti, Lutz Hamann, Hans-Dieter Flad, and Artur J. Ulmer. Matrix-Assisted Laser Desorption/ Ionization Time-of-Flight Mass Spectrometry of Lipopolysaccharides, Buko Lindner. Applications of Combined Capillary Electrophoresis-Electrospray Mass Spectrometry in the Characterization of Short-Chain Lipopolysaccharides: Haemophilus influenzae, Pierre Thibault and James C. Richards. Deacylation of Lipopolysaccharides and Isolation of Oligosaccharide Phosphates, Otto Holst. Electrophysiological Measurements on Reconstituted Outer Membranes, Andre Wiese and Ulrich Seydel. Index.


"The editor has put together an outstanding text on the current protocols used in the study of protein toxins. The caliber of the contributors is outstanding and represents an international perspective. While the editor does not present the text as an exhaustive discussion of microbial toxins, there is a focus on those areas where there is current and active research such as toxin-receptor kinetics, the use of fluorescence resonance energy transfer to detect structural changes in protein toxins, and CE-ESMS of short-chain lipopolysaccharides in Haemophilus influenzae. The organization and content level of this manual makes it a highly desirable addition to either a personal or a departmental library. "-Doody's Health Science Book Review Journal
EAN: 9780896036048
ISBN: 0896036049
Untertitel: Methods and Protocols. 'Methods in Molecular Biology'. 2000. Auflage. Sprache: Englisch.
Erscheinungsdatum: März 2000
Seitenanzahl: 379 Seiten
Format: gebunden
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